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Grasa de sobrepeso en ovejas con diferente espesor de grasa dorsal y su respuesta en el estro sincronizado, perfil endocrino, porcentaje de gestación y prolificidad. Colegio de Postgraduados
Nieto Aquino, Rafael.
El objetivo de este estudio fue evaluar el efecto de la grasa de sobrepaso en el estro sincronizado, porcentaje de gestación y prolificidad en ovejas con baja y alta grasa dorsal, y su respuesta en hormona luteinizante (LH), estradiol (E2), progesterona (P4) e insulina (INS). Se utilizaron 59 ovejas, con diferente espesor de grasa dorsal, bajo (EGb) y alto (EGa), que se subdividieron en grupos: sin (EGbSg, n = 16) y con (EGbCg, n = 14) la adición de 150 g de grasa de sobrepaso; y sin (EGaSg, n = 14) y con (EGaCg, n = 15) la adición de 150 g de grasa de sobrepaso, respectivamente. Se sincronizaron con esponjas de FGA (20 mg), por 12 d, a diez días de su inserción se aplicaron 15 mg de PGF2α. No se encontraron diferencias (P > 0.05) para las...
Tipo: Tesis Palavras-chave: Ultrasonografía; Insulina; Progesterona; Hormona luteinizante.; Maestría; Ganadería; Ultrasonagraphy; Insulin; Progesterone; Estradiol; Luteinizing hormone..
Ano: 2009 URL: http://hdl.handle.net/10521/1210
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Histologic distribution of insulin and glucagon receptors BJMBR
Watanabe,M.; Hayasaki,H.; Tamayama,T.; Shimada,M..
Insulin and glucagon are the hormonal polypeptides secreted by the B and A cells of the endocrine pancreas, respectively. Their major physiologic effects are regulation of carbohydrate metabolism, but they have opposite effects. Insulin and glucagon have various physiologic roles, in addition to the regulation of carbohydrate metabolism. The physiologic effects of insulin and glucagon on the cell are initiated by the binding of each hormone to receptors on the target cells. Morphologic studies may be useful for relating biochemical, physiologic, and pharmacologic information on the receptors to an anatomic background. Receptor radioautography techniques using radioligands to label specific insulin and glucagon receptors have been successfully applied to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Radioautography; Insulin; Glucagon; Receptor; Distribution.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200008
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Immune response, blood parameters and growth performance in broiler fed reduced protein diet supplemented with β-hydroxy-β-methyl butyrate and conjugated linoleic acid Animal Sciences
Saki, Ali Asghar; Khoramabadi, Vahid; Nourian, Ali Reza; Zamani, Pouya.
  In this experiment a total of 300 d-old broilers (Ross 308) were randomized across 20 floor pens and reared up to 42 d. Treatments includes: 1) A normal-protein diet (NPD) was formulated according to the Ross 308 recommendation (2014) by corn and soybean meal, 2) A reduced-protein diet (RPD) was prepared with dietary protein reduced by 12 g kg-1 (1.2%) relative to the NPD, 3) HMB+ RDP, 4) CLA+RDP and 5) CLA+ HMB+ RDP. The results showed that broilers fed HMB diet had higher Average Daily Gain (ADG) and Feed Conversion Rate (FCR) significantly improved by HMB during the grower or the whole period (p < 0.05). Titer of serum antibodies of Newcastle disease was maximum in treatment supplemented with CLA+HMB compared to the control (RPD) in day 42. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hematological parameter; Insulin; Blood cell; Low protein diet; Protein synthesis..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/38000
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Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets Biol. Res.
CUNHA,DANIEL A; AMARAL,MARIA EC; CARVALHO,CAROLINA PF; COLLARES-BUZATO,CARLA B; CARNEIRO,EVERARDO M; BOSCHERO,ANTONIO C.
During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic &#946;-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and...
Tipo: Journal article Palavras-chave: Insulin; Pancreatic islets; Pregnant rats; Prolactin; SNARE proteins; Synaptotagmin.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016
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Influencia de la nutrición en los programas de sincronización de estros, superovulación y transferencia embrionaria en ovejas. Colegio de Postgraduados
Molina Mendoza, Pedro.
El aspecto nutricional y endocrino tiene influencia en la sincronización de estros y programas de superovulación, evaluándose su efecto en dos experimentos. En el experimento 1 se evaluaron dos régimenes alimenticios y dos programas de sincronización de estros, teniendo los siguientes tratamientos: ovejas sin pre-sincronización alimentadas con heno de avena (HS) o concentrado comercial (TS) y pre-sincronizadas con heno de avena (HP), o concentrado comercial (TP). En el experimento 1 la concentración de P4 fue mayor en grupos HP y TP (P<0.05). El inicio del estro y la elevación de LH fueron más rápido en los grupos HS y HP (P<0.05). En el experimento 2 se evaluaron dos régimenes de alimentación, un grupo alimentado con concentrado comercial (T) y...
Palavras-chave: Ovis aries; Grasa dorsal; Superovulación; Progesterona; Insulina; Backfat; Superovulation; Progesterone; Insulin; Doctorado; Ganadería.
Ano: 2010 URL: http://hdl.handle.net/10521/96
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Influencia de la nutrición en los programas de sincronización de estros, superovulación y transferencia embrionaria en ovejas. Colegio de Postgraduados
Molina Mendoza, Pedro.
El aspecto nutricional y endocrino tiene influencia en la sincronización de estros y programas de superovulación, evaluándose su efecto en dos experimentos. En el experimento 1 se evaluaron dos régimenes alimenticios y dos programas de sincronización de estros, teniendo los siguientes tratamientos: ovejas sin pre-sincronización alimentadas con heno de avena (HS) o concentrado comercial (TS) y pre-sincronizadas con heno de avena (HP), o concentrado comercial (TP). En el experimento 1 la concentración de P4 fue mayor en grupos HP y TP (P<0.05). El inicio del estro y la elevación de LH fueron más rápido en los grupos HS y HP (P<0.05). En el experimento 2 se evaluaron dos régimenes de alimentación, un grupo alimentado con concentrado comercial (T) y...
Palavras-chave: Ovis aries; Grasa dorsal; Superovulación; Progesterona; Insulina; Backfat; Superovulation; Progesterone; Insulin; Doctorado; Ganadería.
Ano: 2010 URL: http://hdl.handle.net/10521/96
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Insulin effect during a Heatsynch protocol in dairy cows Ciência Rural
Maffi,Andressa Stein; Brauner,Cássio Cassal; Mielke,Lais Fernanda; Lima,Marcio Erpen; Xavier,Eduardo Gularte; Pino,Francisco Augusto Burkert Del; Corrêa,Marcio Nunes.
ABSTRACT: The objective of this study was to evaluate the effect of exogenous insulin administration on follicular growth, estrus presentation and conception rate during a protocol of ovulation synchronization. Dairy cows were subjected to the Heatsynch protocol, with the insertion of an intravaginal device containing 1.9 g of progesterone (CIDR) and an intramuscular injection containing 2.5 mg of GnRH on day 0. On day 7, the CIDR was removed and subjects were given 12.5 mg of dinoprost. Also on day 7, Insulin Group (IG, n = 21) animals received a subcutaneous injection containing 0.25 IU / kg of recombinant human insulin and the control group (CG, n = 25) received a 0.9% NaCl injection. On day 8, an injection of 0.5 mg of estradiol cypionate was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dairy cow; Insulin; Ovarian follicle; Reproduction.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800650
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Insulin impairs the maturation of chondrocytes in vitro BJMBR
Torres,E.S.; Andrade,C.V.; Fonseca,E.C.; Mello,M.A.; Duarte,M.E.L..
The precise nature of hormones and growth factors directly responsible for cartilage maturation is still largely unclear. Since longitudinal bone growth occurs through endochondral bone formation, excess or deficiency of most hormones and growth factors strongly influences final adult height. The structure and composition of the cartilaginous extracellular matrix have a critical role in regulating the behavior of growth plate chondrocytes. Therefore, the maintenance of the three-dimensional cell-matrix interaction is necessary to study the influence of individual signaling molecules on chondrogenesis, cartilage maturation and calcification. To investigate the effects of insulin on both proliferation and induction of hypertrophy in chondrocytes in vitro we...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chondrocytes; Insulin; Micromass cultures; Apoptosis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900007
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Insulin or insulin-like studies on unicellular organisms: a review BABT
Souza,Alzira Martins Ferreira de; López,Jorge A..
This paper presents a review of studies about insulin and insulin-like substances in prokaryotes, eukaryotes and fungi have been published over the last two decades, constituting an updating of our previous review (1988) which included references to invertebrate insulin or insulin-like substances both in uni- and pluricellular Monera, Protoctista, Fungii and Animal species. This present article reviews experiments and evidence obtained using modern techniques in the understanding of molecule evolution and behaviour, which confirm its very ancient molecular structure origin. The involvement of insulin-like and related material in signalling biological pathway and modulator effects is also reported.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Unicellular; Invertebrate; Insulin; Insulin-like; Insulin receptor.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600017
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Insulin Promotes the Expression of the Gluconeogenic Rate-Limiting Enzymes Phosphoenolpyruvate Carboxykinase (Pepck) and Glucose 6-Phosphatase (G6pase) through PI3k/Akt/mTOR Signaling Pathway in Goose Hepatocytes Rev. Bras. Ciênc. Avic.
Xiong,XP; Song,Q; Han,CC; Gan,W; He,F; Wei,SH; Liu,HH; Xu,HY.
ABSTRACT To identify what makes insulin have an activating or inhibiting role in gluconeogenesis in goose hepatocytes and whether insulin regulates PEPCK and G6Pase through the PI3k/Akt/mTOR pathway or not, goose primary hepatocytes were isolated and cultured in vitro. After 12h cultured in serum-free medium, hepatocytes were incubated for 24 h in the medium with no addition (control) or with the addition of50, 100, and 150 nM of insulin, 1000 nM NVP-BEZ235, or co-addition of 150nM insulin and 1000nM NVP-BEZ235. Glucose concentration and PEPCK and G6Pase expression were determined. The results showed that PEPCK and G6Pase mRNA levels and activities were up regulated in the 50, 100, and 150nM insulin treatments, while glucose concentration was not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: G6Pase; Insulin; PEPCK; PI3k/Akt/mTOR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300395
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Insulin-loaded polymeric mucoadhesive nanoparticles: development, characterization and cytotoxicity evaluation BJPS
Gatti,Tiago Henrique Honorato; Eloy,Josimar Oliveira; Ferreira,Leonardo Miziara Barboza; Silva,Isabel Cristine da; Pavan,Fernando Rogério; Gremião,Maria Palmira Daflon; Chorilli,Marlus.
Abstract Mucoadhesive nanoparticles are particularly interesting for delivery through nasal or pulmonary routes, as an approach to overcome the mucociliary clearance. Moreover, these nanoparticles are attractive for peptide and protein delivery, particularly for insulin to treat diabetes, as an alternative to conventional parenteral administration. Thus, chitosan, a cationic mucoadhesive polysaccharide found in shells of crustaceans, and the negatively-charged dextran sulfate are able to form nanoparticles through ionic condensation, representing a potential insulin carrier. Herein, chitosan/dextran sulfate nanoparticles at various ratios were prepared for insulin loading. Formulations were characterized for particle size, zeta potential, encapsulation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin; Chitosan; Dextran-Sulfate; Nanoparticles; Mucoadhesion..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100623
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Leptin is not associated independently with hypertension in Japanese-Brazilian women BJMBR
Almeida-Pititto,B.; Gimeno,S.G.A.; Freire,R.D.; Ribeiro-Filho,F.F.; Ferreira,S.R.G..
We evaluated the relationship of leptin with hypertension adjusted for body mass index (BMI) and/or waist circumference in a population of Japanese-Brazilian women aged > or = 30 years with centrally distributed adiposity. After excluding diabetic subjects, the study subjects - who participated in a population-based study on the prevalence of metabolic syndrome - showed prevalence rates of obesity (BMI > or = 25 kg/m²) and central adiposity (waist > or = 80 cm) of 32.0 and 37.8%, respectively. The hypertensive group (N = 162) was older, had higher BMI (24.9 ± 4.2 vs 23.3 ± 3.4 kg/m², P < 0.001), waist circumference (81.1 ± 10.1 vs 76.3 ± 8.2 cm, P < 0.001) and insulin levels (8.0 ± 6.2 vs 7.1 ± 4.9 µU/mL, P < 0.05) than the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypertension; Body fat distribution; Insulin; Leptin; Centrally obese; Metabolic syndrome.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100012
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Linking dietary energy and skeletal development in the horse R. Bras. Zootec.
Staniar,William Burton.
Athletic production is what is sought from the horse. As mammary development is important to the dairy cow, skeletal development is important to horses meeting their production goals. As any integrative physiologist will appreciate, the variables that come together to result in optimal skeletal development are complex. Nutrition is one of these, and it contains two broad variables; the supply of dietary nutrients and energy. This presentation will focus on dietary energy and its links with skeletal development. I propose that it is not simply the amount of dietary energy, but the way and from that that energy is supplied that impacts skeletal development. Through an understanding of how dietary energy impact skeletal development, more precise feeding...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cartilage; Equine; Glucose; Growth; Insulin; Somatotropic axis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300016
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Low levels of sex hormone-binding globulin and hyperproinsulinemia as markers of increased pancreatic ß-cell demand in men BJMBR
Reis,A.F.; Miranda,W.L.; Chacra,A.R..
Low levels of sex hormone-binding globulin (SHBG) are considered to be an indirect index of hyperinsulinemia, predicting the later onset of diabetes mellitus type 2. In the insulin resistance state and in the presence of an increased pancreatic ß-cell demand (e.g. obesity) both absolute and relative increases in proinsulin secretion occur. In the present study we investigated the correlation between SHBG and pancreatic ß-cell secretion in men with different body compositions. Eighteen young men (30.0 ± 2.4 years) with normal glucose tolerance and body mass indexes (BMI) ranging from 22.6 to 43.2 kg/m2 were submitted to an oral glucose tolerance test (75 g) and baseline and 120-min blood samples were used to determine insulin, proinsulin and C-peptide by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin; Proinsulin; Sex hormone-binding globulin; Obesity.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200006
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Malnutrition during lactation as a metabolic imprinting factor inducing the feeding pattern of offspring rats when adults: The role of insulin and leptin BJMBR
Moura,A.S.; Franco de Sá,C.C.N.; Cruz,H.G.; Costa,C.L..
The aim of the present study was to determine the impact of malnutrition during early postnatal life and the feeding pattern of rat offspring when adults (2 months and 1 year old). In comparison with rats normally fed during lactation, we observed that adult offspring displayed a faster process of feeding reduction when a protein-free diet was offered. In addition, we studied the concentration of insulin and leptin in the lactating pups (10 days) and when these offspring became adult after the onset of a new feeding pattern induced by the protein-free diet. When the diet was changed at 60 days, the offspring malnourished during lactation displayed, after 3 days, a food intake reduction around 41.4 vs 14.2% of the control group. At 10 days of life, plasma...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nutrition; Growth; Insulin; Leptin.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500016
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Metabolic dysregulation in early onset psychiatric disorder before and after exposure to antipsychotic drugs BJPS
Shah,Rehmat; Subhan,Fazal; Sultan,Syed Muhammad; Haq,Matiul; Ahmad,Hussain; Khan,Qasim Riaz; Ali,Gowhar; Ullah,Sami; Ullah,Ihsan.
Antipsychotic Drugs (APDs) are being widely prescribed to treat various disorders, including schizophrenia and bipolar disorder; however, abnormal glucose metabolism and weight gain have been reported with Atypical Anti-Psychotic drugs (AAPDs) that can lead to insulin-resistance and type 2 diabetes mellitus. The study was designed to assess various biochemical parameters including insulin and blood sugar before and after exposure to APDs in order to exclude the involvement of psychiatric disorders and certain other factors in metabolic dysregulations. Fifty seven APDs-naïve patients with first episode psychosis were divided into six groups who received olanzapine, quetiapine, risperidone, aripiprazole, haloperidol or combination of olanzapine with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Olanzapine; Quetiapine; Risperidone; Antipsychotics; Pancreas; Insulin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100535
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Milk production in Saanen goats treated with repeated low doses of intermediate-release insulin during early lactation Ciência Rural
Menezes,Erika Bezerra de; Salles,Maria Gorete Flores; Silva,Cleidson Manoel Gomes da; Fernandes,César Carneiro Linhares; Galeati,Giovanna; Araújo,Airton Alencar de; Rondina,Davide.
ABSTRACT: The effect of insulin administration on the productive responses of Saanen goats during early lactation was investigated. Ten of 20 adult females were subjected to subcutaneous administration of intermediate-acting insulin (0.14UI/kg body weight) at 2, 9, and 14 days postpartum. Milk yield was measured twice daily for 13 weeks and milk samples were collected to measure protein and fat contents. Plasma levels of progesterone, insulin, non-esterifies fatty acids, glucose and other metabolites were measured. Results showed a significantly increased effect of insulin treatment on the content of milk fat and protein; moreover, milk production in the first and second postpartum weeks were higher than control group. The peak of lactation in the insulin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Insulin; Milk; Postpartum; Metabolites.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300652
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Modulation of hormone secretion by functional electrical stimulation of the intact and incompletely dysfunctional dog pancreas BJMBR
Rozman,J.; Bunc,M.; Zorko,B..
The purpose of the present study was to modulate the secretion of insulin and glucagon in Beagle dogs by stimulation of nerves innervating the intact and partly dysfunctional pancreas. Three 33-electrode spiral cuffs were implanted on the vagus, splanchnic and pancreatic nerves in each of two animals. Partial dysfunction of the pancreas was induced with alloxan. The nerves were stimulated using rectangular, charge-balanced, biphasic, and constant current pulses (200 µs, 1 mA, 20 Hz, with a 100-µs delay between biphasic phases). Blood samples from the femoral artery were drawn before the experiment, at the beginning of stimulation, after 5 min of stimulation, and 5 min after the end of stimulation. Radioimmunoassay data showed that in the intact pancreas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Functional electrical stimulation; Implanted electrodes; Autonomic nerves; Pancreatic secretion; Insulin; Glucagon.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300012
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Neutrophil function and metabolism in individuals with diabetes mellitus BJMBR
Alba-Loureiro,T.C.; Munhoz,C.D.; Martins,J.O.; Cerchiaro,G.A.; Scavone,C.; Curi,R.; Sannomiya,P..
Neutrophils act as first-line-of-defense cells and the reduction of their functional activity contributes to the high susceptibilityto and severity of infections in diabetes mellitus. Clinical investigations in diabetic patients and experimental studies in diabetic rats and mice clearly demonstrated consistent defects of neutrophil chemotactic, phagocytic and microbicidal activities. Other alterations that have been reported to occur during inflammation in diabetes mellitus include: decreased microvascular responses to inflammatory mediators such as histamine and bradykinin, reduced protein leakage and edema formation, reduced mast cell degranulation, impairment of neutrophil adhesionto the endothelium and migration to the site of inflammation, production...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neutrophils; Inflammation; Diabetes mellitus; Insulin; Metabolism and cellular function.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800003
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Oocyte production in Nellore cows supplemented with long-chain fatty acid soaps R. Bras. Zootec.
Souza,Moacir Rogério de; Santos,Geraldo Tadeu dos; Lima,Luciano Soares de; Mazucheli,Josmar; Zeoula,Lúcia Maria; Rigolon,Luiz Paulo; Cavalieri,Fábio Luiz Bim.
ABSTRACT This study was conducted to evaluate the effects of long-chain fatty acid soaps (LCFAS) of soybean oil on the production and quality of aspirated cumulus-oocyte complexes (COC) of lactating Nellore (Bos taurus indicus) cows. The effects of LCFAS on the plasma concentration of lipoproteins, total cholesterol, triacylglycerol, urea N, and insulin were also addressed. Thirty lactating Nellore cows were randomly assigned to one of two dietary groups. Cows were grazed on a Tifton 85 pasture throughout the experiment (100 days). Treatments consisted of a supplement with no fat included (CONT); and a supplement containing 47.2 g/kg of LCFAS on a dry matter basis. After 14 days of treatment, animals were subjected to 4.93±1.55 rounds of consecutive ovum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef cattle; Insulin; Fat supplementation; Lipid profile; Ovum pick up.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000300138
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